Publications on ResearchGate:
Daniel E. Callahan (Publications on ResearchGate)
Coupling Computers to Living Cells for Automated Knowledge Extraction
December 07-December 12
eScience, 2008. eScience apos;08. IEEE Fourth International Conference on
Volume , Issue , 7-12 Dec. 2008 Page(s):446 - 447
Digital Object Identifier 10.1109/eScience.2008.152
Abstract: A general method for coupling individual living cells to computers is presented. This method is called visual-servoing optical microscopytrade (VSOMtrade). Protocols, instrumentation, and software are described and experimental results are presented. During VSOM experiments, automated decisions are made as single cell responses to computer-applied stimuli are interpreted in real-time. Well-annotated live cell responses are stored in distributed VSOM databases in real-time. This closed-loop method enables automated extraction of knowledge from individual living cells (click here to read more).
BioSig: An Informatics Framework for Representing the Physiological Responses of Living Cells
B. Parvin, and D.E. Callahan, BioSig: An Informatics Framework for Representing the Physiological Responses of Living Cells, BioSilico, 1: 42-46 (2003).
Abstract: Subcellular experimental datasets and detailed cell models are required before modeling of whole organs. Cell modeling requires repeated interaction between simulation and experimental data. This review describes a coupled system of informatics and instrument control suitable for extracting information at the subcellular level. The BioSig informatics framework annotates time-series images with experimental variables and computed representation such as physiological responses, whereas visual servoing optical microscopy (VSOM) is used for adaptive perturbation and interaction with living cells ( click here to read more).